Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.683

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	7.31E-02	7.37E-02	7.28E-02
	B	25.00	6.12E-02	6.89E-02	6.14E-02
	C	8.33	5.87E-02	5.61E-02	5.45E-02
	D	2.78	3.20E-02	3.08E-02	3.33E-02
	E	0.93	1.40E-02	1.37E-02	1.46E-02
	F	0.31	5.70E-03	5.40E-03	5.25E-03
	G	0.10	2.25E-03	1.95E-03	2.70E-03
	H	0.03	0	0	0

Yield (mg/mL): 1.683     Dilution Factor:  100×

Download these data

Protein purified on 3/24/23 and assayed on 3/31/23.
Data uploaded by murigs and last updated on 2023-04-14 07:07:37.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04	4.50E-04	9.00E-04
	B	48.3
	C	45.7	9.00E-04	9.00E-04	7.50E-04
	D	42.4	3.75E-03	3.75E-03	3.45E-03
	E	37.7	1.64E-02	1.73E-02	1.65E-02
	F	33.6	2.19E-02	2.33E-02	2.19E-02
	G	31.3	2.33E-02	2.28E-02	2.16E-02
	H	30.0	2.04E-02	2.19E-02

Download these data

Protein purified on 3/24/2023 and assayed on 3/31/2023.
Data uploaded by murigs and last updated on 2024-05-23 09:39:36.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.