Raw Data for BglB Variant W409H

Expression Data

SDS-PAGE
 0.72

Kinetic Assay Data

slope of W409H A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.79E-02 7.92E-02 8.22E-02
B 25.00 8.03E-02 7.15E-02 7.47E-02
C 8.33 6.48E-02 5.88E-02
D 2.78 4.43E-02 3.95E-02 3.89E-02
E 0.93 1.84E-02 1.92E-02 1.94E-02
F 0.31 8.10E-03 7.80E-03 7.50E-03
G 0.10 2.70E-03 3.20E-03 2.60E-03
H 0.03 4.00E-04 4.00E-04
 0.72      100×

Protein purified on 4/11/2024 and assayed on 4/18/2024.
Data uploaded by RubenR98 and last updated on 2024-05-16 11:46:45.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.18E-01 1.17E-01 1.10E-01
B 25.00 1.01E-01 8.86E-02 8.72E-02
C 8.33 6.06E-02 5.76E-02 4.79E-02
D 2.78 2.94E-02 2.96E-02 2.81E-02
E 0.93 1.13E-02 1.09E-02 1.09E-02
F 0.31 4.00E-03 3.90E-03 3.60E-03
G 0.10 1.50E-03 1.60E-03 1.50E-03
H 0.03 0.00E+00 5.00E-04 8.00E-04
 0.752      100×

Protein purified on 4/11/2024 and assayed on 4/20/2024.
Data uploaded by KeairaC and last updated on 2024-05-16 11:30:43.


Thermostability Assay Data

slope of W409H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0
B 48.3 0.0006001 0.0007
C 45.7 0.0008 0.0003 0.0005
D 42.4 0.0232 0.0207 0.0158
E 37.7 0.1386 0.1435 0.1277
F 33.6 0.3154
G 31.3 0.1411 0.1412 0.1464
H 30.0 0.1429 0.147 0.1403

Protein purified on 11-Apr and assayed on 20-Apr.
Data uploaded by Ericacua and last updated on 2024-05-17 08:59:31.


Reference Data for WT

slope of W409H A420 nm (min−1)
T (°C) 1 2 3
A 50.0 5.00E-04 6.00E-04
B 48.3 3.00E-04 0.00E+00 4.00E-04
C 45.7 5.00E-04 6.00E-04 7.00E-04
D 42.4 2.66E-02 2.65E-02 3.02E-02
E 37.7 1.36E-01 1.31E-01 1.31E-01
F 33.6 1.40E-01 1.36E-01
G 31.3 1.38E-01 1.35E-01 1.34E-01
H 30.0 1.44E-01 1.44E-01

Protein purified on 4/11/2024 and assayed on 4/20/2024.
Data uploaded by KeairaC and last updated on 2024-05-16 11:40:39.


Design Data

2.123

Citations

The unpublished data above were collected by students from MiraCosta College. For inquiries, please contact Suzie Bailey: <sbailey@miracosta.edu>.