D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D63S

Expression Data

Yield (mg/mL): 0.347

Kinetic Assay Data

**slope of D63S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0	0
B	25.00		0	0
C	8.33	0.0002	0.0001	0.00015
D	2.78	0.00025	0.0002	0.0002
E	0.93	0.00015	0.00015
F	0.31	0.00015	0.0001	0.00025
G	0.10	0	0	0
H	0.03	0.00015	0	0.0002

Yield (mg/mL): 0.347 Dilution Factor: 10×

Protein purified on 5/18/22 and assayed on 5/19/22.
Data uploaded by viv513 and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.07125	0.0719	0.0822
B	25.00	0.06545	0.07175	0.07545
C	8.33	0.04625	0.0488	0.0454
D	2.78	0.0247	0.027	0.02715
E	0.93	0.0104	0.01155	0.01025
F	0.31	0.00275	0.00315	0.00335
G	0.10	0.00115	0.0009	0.00155
H	0.03	0.0005	0.0006

Yield (mg/mL): 1.066 Dilution Factor: 100×

Protein purified on 5/18/22 and assayed on 5/19/22.
Data uploaded by eleanorsmith and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of D63S A_{420 nm}**
	T (°C)	1	2	3
A	50.0	0.0007	0.0005	0.0004
B	48.3	0.0005		0.0004
C	45.7		0.0009	0.001
D	42.4	0.0545	0.0568	0.0663
E	37.7	0.3156	0.3369	0.3544
F	33.6	0.3311	0.3566	0.3662
G	31.3	0.3741	0.3486	0.3749
H	30.0	0.2971	0.3295	0.3265

Protein purified on 5/18/22 and assayed on 5/19/22.
Data uploaded by eleanorsmith and last updated on 2022-06-27 07:10:33.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.