Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.27

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.87E-01	1.33E-01	1.64E-01
	B	25.00	1.38E-01	1.70E-01	1.53E-01
	C	8.33		6.64E-02	7.01E-02
	D	2.78	3.26E-02	3.58E-02	3.23E-02
	E	0.93		1.95E-02	1.90E-02
	F	0.31	6.70E-03	8.45E-03	5.65E-03
	G	0.10		4.30E-03	4.50E-03
	H	0.03	1.70E-03	1.60E-03

Yield (mg/mL): 2.27     Dilution Factor:  100×

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Protein purified on 5/18/22 and assayed on 5/19/22.
Data uploaded by sdas407 and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm}

		T (°C)	1	2	3
	A	50.0	1.70E-03	9.00E-04	6.00E-04
	B	48.0	6.00E-04	7.00E-04
	C	46.0	3.40E-03	4.70E-03	3.80E-03
	D	42.0	3.06E-01	2.73E-01	3.43E-01
	E	38.0	9.57E-01	1.01E+00	9.76E-01
	F	34.0	1.09E+00	1.07E+00	1.10E+00
	G	31.0	9.70E-01	1.01E+00	1.04E+00
	H	30.0	1.06E+00	1.08E+00	1.10E+00

Download these data

Protein purified on 5/18/22 and assayed on 5/19/22.
Data uploaded by sdas407 and last updated on 2022-06-27 07:10:33.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.