D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D63N

Yield (mg/mL): 0.442

**slope of D63N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0002500	0.0003500	0.0002000
B	25.00	0.0001500	0.0002000	0
C	8.33	0	0
D	2.78	0.0003500	0.0007500	0.0002000
E	0.93		0	0
F	0.31	0.0002000	0.0001500	0.0001500
G	0.10	0	0	0
H	0.03	0	0

Yield (mg/mL): 0.442 Dilution Factor: 10×

Protein purified on 5/18/2022 and assayed on 5/19/2022.
Data uploaded by Prsiu and last updated on 2022-06-27 07:04:03.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.07125	0.0719	0.0822
B	25.00	0.06545	0.07175	0.07545
C	8.33	0.04625	0.0488	0.0454
D	2.78	0.0247	0.027	0.02715
E	0.93	0.0104	0.01155	0.01025
F	0.31	0.00275	0.00315	0.00335
G	0.10	0.00115	0.0009	0.00155
H	0.03	0.0005	0.0006

Yield (mg/mL): 1.066 Dilution Factor: 100×

Protein purified on 5/18/22 and assayed on 5/19/22.
Data uploaded by eleanorsmith and last updated on 2022-06-27 07:04:03.

**slope of D63N A_{420 nm}**
		T (°C)	1	2	3
	A	30.0	0	0	0
	B