D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R76Q

Expression Data

Yield (mg/mL): 0.703

Kinetic Assay Data

**slope of R76Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.002000	0.001550	0.001750
B	25.00	0.001400	0.001150	0.001150
C	8.33	0.0009500	0.0008000	0.0008000
D	2.78	0.0007000	0.0005500	0.0005000
E	0.93	0.0004000	0.0004000	0.0004000
F	0.31	0.0003000	0.0002500	0.0003500
G	0.10	0.0004000	0.0002500	0.0001500
H	0.03	0.0002000	0.0006500	0.0004500

Yield (mg/mL): 0.703 Dilution Factor: 10×

Protein purified on 4/19/22 and assayed on 4/20/22.
Data uploaded by eandrejr and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2586	0.2452	0.2764
B	25.00	0.2196	0.2239	0.2194
C	8.33	0.1850	0.1716	0.1921
D	2.78	0.1039	0.1057	0.1200
E	0.93	0.07245	0.07070	0.06070
F	0.31	0.03155	0.03105	0.03710
G	0.10	0.01520	0.01295	0.01200
H	0.03	0.0003000	0.0001500	0.0003500

Yield (mg/mL): 3.809 Dilution Factor: 100×

Protein purified on 4/19/22 and assayed on 4/20/22.
Data uploaded by eandrejr and last updated on 2022-06-27 07:04:03.

Design Data

Rosetta Score for R76Q: -0.07

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.