D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q313C

Expression Data

Yield (mg/mL): 4.678

Kinetic Assay Data

**slope of Q313C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5245	0.5393	0.5319
B	25.00	0.4243	0.4410	0.4327
C	8.33	0.3274	0.2971	0.3125
D	2.78	0.1826	0.1809	0.1818
E	0.93	0.07840	0.08045	0.07997
F	0.31	0.02515	0.02975	0.02745
G	0.10	0.009750	0.009951	0.009851
H	0.03	0.0009500	0.0002500	0.0006700

Yield (mg/mL): 4.678 Dilution Factor: 100×

Protein purified on 6/2/2022 and assayed on 6/3/2022.
Data uploaded by cgyeow and last updated on 2022-06-27 07:04:03.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4766	0.4738	0.4752
B	25.00	0.3862	0.3683	0.3779
C	8.33	0.2816	0.2799	0.2808
D	2.78	0.1566	0.1498	0.1532
E	0.93	0.06110	0.06675	0.06396
F	0.31	0.02245	0.02340	0.02293
G	0.10	0.01025	0.007901	0.009076
H	0.03		0.0002500	0.0002500

Yield (mg/mL): 3.746 Dilution Factor: 100×

Protein purified on 6/2/2022 and assayed on 6/3/2022.
Data uploaded by cgyeow and last updated on 2022-06-27 07:04:03.

Design Data

Rosetta Score for Q313C: 0.926

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.