D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W409E

Expression Data

Yield (mg/mL): 2.591

Kinetic Assay Data

**slope of W409E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.32E-01	2.44E-01	2.54E-01
B	25.00	1.81E-01	2.18E-01	2.09E-01
C	8.33	2.31E-01	2.09E-01	1.84E-01
D	2.78	1.42E-01	1.46E-01	1.25E-01
E	0.93	7.60E-02	7.47E-02	7.03E-02
F	0.31	3.24E-02	3.02E-02	3.74E-02
G	0.10		1.14E-02	9.65E-03
H	0.03	2.50E-04	3.00E-04	3.50E-04

Yield (mg/mL): 2.591 Dilution Factor: 100×

Protein purified on 5/11/22 and assayed on 5/11/22.
Data uploaded by wateraus and last updated on 2022-06-30 16:46:04.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.81E-01	1.99E-01	1.87E-01
B	25.00	1.52E-01	1.20E-01	1.77E-01
C	8.33	1.18E-01	1.45E-01	1.04E-01
D	2.78	9.41E-02	9.41E-02	7.95E-02
E	0.93	3.78E-02	4.10E-02	3.09E-02
F	0.31	1.36E-02	1.65E-02	1.40E-02
G	0.10	5.30E-03	4.45E-03	5.15E-03
H	0.03	2.50E-04	2.50E-04

Yield (mg/mL): 1.76 Dilution Factor: 100×

Protein purified on 4/18/22 and assayed on 4/20/22.
Data uploaded by bridget5 and last updated on 2023-01-11 14:39:36.

Design Data

Rosetta Score for W409E: 4.878

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.