D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Y166K

Expression Data

Yield (mg/mL): 0.338

Kinetic Assay Data

**slope of Y166K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.001850	0.001600	0.001800
B	25.00	0.001600	0.001750	0.001750
C	8.33	0.001350	0.001550	0.001400
D	2.78	0.0009000	0.0008500	0.0007500
E	0.93	0.0007500	0.0005000	0.0006000
F	0.31		0.0003500	0.0003000
G	0.10	0.0004000		0.0004500
H	0.03	0.0004500		0.0004501

Yield (mg/mL): 0.338 Dilution Factor: 10×

Protein purified on 6/30/2022 and assayed on 7/5/2022.
Data uploaded by morgan55 and last updated on 2022-07-06 15:38:08.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5403	0.5610	0.5446
B	25.00	0.5036	0.5109	0.4649
C	8.33	0.3866	0.3866	0.3604
D	2.78	0.2796	0.2790	0.2534
E	0.93	0.1473	0.1326	0.1282
F	0.31	0.05265	0.04870	0.04480
G	0.10	0.01970	0.01850	0.01385
H	0.03	0.007100	0.007150	0.006650

Yield (mg/mL): 2.96 Dilution Factor: 100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:04:03.

Design Data

Rosetta Score for Y166K: 11.356

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.