D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant V311D

Expression Data

Yield (mg/mL): 3.048

Kinetic Assay Data

**slope of V311D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2179	0.2463	0.2747
B	25.00	0.1970	0.2177	0.2136
C	8.33	0.1390	0.1286	0.1508
D	2.78	0.07890	0.08725	0.08035
E	0.93	0.03405	0.03715	0.03440
F	0.31	0.01290	0.01480	0.01765
G	0.10	0.004700	0.005250	0.006350
H	0.03	0.001650	0.002950	0.002500

Yield (mg/mL): 3.048 Dilution Factor: 100×

Protein purified on 6/30/2022 and assayed on 7/5/2022.
Data uploaded by morgan55 and last updated on 2022-07-06 15:50:31.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.5403	0.5610	0.5446
B	25.00	0.5036	0.5109	0.4649
C	8.33	0.3866	0.3866	0.3604
D	2.78	0.2796	0.2790	0.2534
E	0.93	0.1473	0.1326	0.1282
F	0.31	0.05265	0.04870	0.04480
G	0.10	0.01970	0.01850	0.01385
H	0.03	0.007100	0.007150	0.006650

Yield (mg/mL): 2.96 Dilution Factor: 100×

Protein purified on 11/4/2021 and assayed on 11/18/2021.
Data uploaded by kuocarys and last updated on 2022-06-27 07:04:03.

Design Data

Rosetta Score for V311D: 2.594

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.