D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N293Y

Expression Data

Yield (mg/mL): 0.606

Kinetic Assay Data

**slope of N293Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.50E-04	9.00E-04	7.50E-04
B	25.00	3.00E-04	4.50E-04	5.00E-04
C	8.33	0		0
D	2.78	0	3.00E-04	4.50E-04
E	0.93		0	0
F	0.31	0	0	0
G	0.10	0	0
H	0.03	0	0

Yield (mg/mL): 0.606 Dilution Factor: 10×

Protein purified on 6/30/2022 and assayed on 7/5/2022.
Data uploaded by scdonohugh and last updated on 2022-07-07 17:18:13.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.95E-01	1.98E-01
B	25.00	1.61E-01	1.73E-01
C	8.33	1.25E-01	1.41E-01
D	2.78	6.27E-02	5.39E-02
E	0.93	2.92E-02	2.95E-02
F	0.31	1.11E-02	1.10E-02
G	0.10	4.55E-03	4.10E-03
H	0.03		0	0

Yield (mg/mL): 2.592 Dilution Factor: 100×

Protein purified on 6/30/2022 and assayed on 7/5/2022.
Data uploaded by scdonohugh and last updated on 2022-07-07 17:11:17.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.