D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N404E

Expression Data

Yield (mg/mL): 1.76

Kinetic Assay Data

**slope of N404E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.004100	0.003950	0.004150
B	25.00	0.002950	0.002950	0.003000
C	8.33	0.001800	0.002050	0.002150
D	2.78	0.001100	0.001250	0.001300
E	0.93	0.0007000		0.0007500
F	0.31	0.0006000	0	0.0004000
G	0.10	0	0.0004000	0.0003000
H	0.03	0		0

Yield (mg/mL): 1.76 Dilution Factor: 10×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by scdonohugh and last updated on 2022-07-13 14:37:38.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2752	0.2637	0.2828
B	25.00	0.2145	0.2203	0.2203
C	8.33	0.1753	0.1578	0.1619
D	2.78	0.1207	0.09175	0.1075
E	0.93	0.05585	0.05140	0.05090
F	0.31	0.01880	0.02055	0.02130
G	0.10	0.007400	0.007050	0.006850
H	0.03	0.0003000		0.0003000

Yield (mg/mL): 2.552 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by scdonohugh and last updated on 2022-07-13 14:43:44.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.