D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N157D

Expression Data

Yield (mg/mL): 0.721

Kinetic Assay Data

**slope of N157D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.02E-01	6.83E-01	6.71E-01
B	25.00	5.25E-01	5.35E-01	5.10E-01
C	8.33	3.80E-01	3.88E-01	3.78E-01
D	2.78	2.54E-01	2.47E-01	2.18E-01
E	0.93	1.26E-01	1.18E-01	1.13E-01
F	0.31	4.23E-02	4.26E-02	4.17E-02
G	0.10	1.49E-02	1.54E-02	1.63E-02
H	0.03	4.50E-04	4.00E-04	4.00E-04

Yield (mg/mL): 0.721 Dilution Factor: 10×

Protein purified on 7/11/22 and assayed on 7/12/22.
Data uploaded by Andrew and last updated on 2022-07-14 15:59:04.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.70E-01	2.75E-01	2.75E-01
B	25.00	2.27E-01	2.21E-01	2.15E-01
C	8.33		1.68E-01	1.82E-01
D	2.78	1.05E-01	1.17E-01	1.29E-01
E	0.93	4.68E-02	4.94E-02	5.03E-02
F	0.31	1.54E-02	1.61E-02	1.65E-02
G	0.10	8.10E-03	6.15E-03	7.15E-03
H	0.03	5.00E-04	5.00E-04	5.00E-04

Yield (mg/mL): 2.723 Dilution Factor: 100×

Protein purified on 7/11/22 and assayed on 7/12/22.
Data uploaded by Andrew and last updated on 2022-07-14 16:12:06.

Design Data

Rosetta Score for N157D: -1.004

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.