D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W407D

Expression Data

Yield (mg/mL): 0.143

Kinetic Assay Data

**slope of W407D A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.00E-01
B	25.00	2.00E-01		2.00E-01
C	8.33	5.90E+00	3.00E-01
D	2.78	2.00E-01	4.00E-01
E	0.93	2.20E+00	1.30E+00	3.00E-01
F	0.31	3.00E-01	3.00E-01
G	0.10	2.00E-01		1.00E-01
H	0.03

Yield (mg/mL): 0.143 Dilution Factor: 10×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by Sarahbob and last updated on 2022-07-15 14:08:47.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.99E+02	3.14E+02	3.10E+02
B	25.00	2.48E+02	2.45E+02	2.42E+02
C	8.33	2.31E+02	1.97E+02	2.11E+02
D	2.78	1.27E+02	1.27E+02	1.16E+02
E	0.93	4.03E+01	5.35E+01	5.93E+01
F	0.31	1.33E+01	2.26E+01	1.68E+01
G	0.10	6.10E+00	6.70E+00	5.70E+00
H	0.03		2.00E-01	2.00E-01

Yield (mg/mL): 2.833 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by Sarahbob and last updated on 2022-07-15 13:31:38.

Design Data

Rosetta Score for W407D: 2.542

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.