D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H419Y

Expression Data

Yield (mg/mL): 3.317

Kinetic Assay Data

**slope of H419Y A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.94E+02	4.10E+02	3.95E+02
B	25.00	2.98E+02	2.82E+02	2.83E+02
C	8.33	2.56E+02	2.60E+02	2.33E+02
D	2.78	1.83E+02	1.94E+02	1.67E+02
E	0.93	8.43E+01	8.24E+01	5.86E+01
F	0.31	3.21E+01	2.97E+01
G	0.10	1.11E+01	1.07E+01
H	0.03			1.00E-01

Yield (mg/mL): 3.317 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by dyoustra and last updated on 2022-07-15 15:25:48.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	60.3	6.53E+01	6.33E+01
B	25.00	45.8	4.56E+01	4.83E+01
C	8.33	4.47E+01	4.57E+01	3.83E+01
D	2.78	2.53E+01	2.81E+01	2.25E+01
E	0.93	1.18E+01	1.31E+01	9.10E+00
F	0.31	4.50E+00	4.50E+00	3.20E+00
G	0.10	1.90E+00	1.90E+00
H	0.03		1.00E-01	1.00E-01

Yield (mg/mL): 0.707 Dilution Factor: 100×

Protein purified on 7/11/2022 and assayed on 7/12/2022.
Data uploaded by dyoustra and last updated on 2022-07-15 14:52:56.

Design Data

Rosetta Score for H419Y: -2.083

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.