D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q139K

Expression Data

Yield (mg/mL): 0.473

Kinetic Assay Data

**slope of Q139K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.04385	0.04365	0.04325
B	25.00	0.0348	0.0328	0.0342
C	8.33	0.02455	0.0241	0.0241
D	2.78	0.01405	0.01535	0.01465
E	0.93	0.00755	0.0079	0.0074
F	0.31	0.003	0.00315	0.0031
G	0.10	0.0013	0.00145	0.0013
H	0.03	0	0.00035	0

Yield (mg/mL): 0.473 Dilution Factor: 10×

Protein purified on 6/7/2022 and assayed on 6/8/2022.
Data uploaded by nikchupkin and last updated on 2022-07-18 20:15:37.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0381	0.03795	0.0369
B	25.00	0.0305	0.02905	0.0265
C	8.33	0.02175	0.0208	0.0203
D	2.78	0.01335	0.0117	0.0122
E	0.93	0.0059	0.006	0.00615
F	0.31	0.00315	0.0027	0.00315
G	0.10	0.00135	0.00105	0.00115
H	0.03	0	0	0

Yield (mg/mL): 0.576 Dilution Factor: 100×

Protein purified on 6/7/2022 and assayed on 6/8/2022.
Data uploaded by nikchupkin and last updated on 2022-07-14 16:58:39.

Design Data

Rosetta Score for Q139K: -3.785

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.