D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K341A

Expression Data

Yield (mg/mL): 3.138

Kinetic Assay Data

**slope of K341A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3211	0.3347	0.337
B	25.00	0.2638	0.2721	0.2618
C	8.33	0.1901	0.2034	0.2046
D	2.78	0.1119	0.1268	0.1277
E	0.93	0.06225	0.07096	0.0648
F	0.31	0.0252	0.03095	0.0257
G	0.10	0.01	0.01185	0.00985
H	0.03	0	0.00075	0

Yield (mg/mL): 3.138 Dilution Factor: 100×

Protein purified on 6/7/2022 and assayed on 6/8/2022.
Data uploaded by nikchupkin and last updated on 2022-07-18 20:18:42.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0381	0.03795	0.0369
B	25.00	0.0305	0.02905	0.0265
C	8.33	0.02175	0.0208	0.0203
D	2.78	0.01335	0.0117	0.0122
E	0.93	0.0059	0.006	0.00615
F	0.31	0.00315	0.0027	0.00315
G	0.10	0.00135	0.00105	0.00115
H	0.03	0	0	0

Yield (mg/mL): 0.576 Dilution Factor: 100×

Protein purified on 6/7/2022 and assayed on 6/8/2022.
Data uploaded by nikchupkin and last updated on 2022-07-14 16:58:39.

Design Data

Rosetta Score for K341A: 2.865

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.