D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R427L

Expression Data

Yield (mg/mL): 0.406

Kinetic Assay Data

**slope of R427L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4496	0.4563	0.4564
B	25.00	0.3532	0.3525	0.3523
C	8.33	0.2689	0.2762	0.2958
D	2.78	0.1698	0.1689	0.175
E	0.93	0.087	0.0846	0.0924
F	0.31	0.0346	0.03315	0.03625
G	0.10	0.014	0.0126	0.0138
H	0.03	0.0004	0.0003	0.0003

Yield (mg/mL): 0.406 Dilution Factor: 10×

Protein purified on 6/7/2022 and assayed on 6/8/2022.
Data uploaded by nikchupkin and last updated on 2022-07-18 20:21:05.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0381	0.03795	0.0369
B	25.00	0.0305	0.02905	0.0265
C	8.33	0.02175	0.0208	0.0203
D	2.78	0.01335	0.0117	0.0122
E	0.93	0.0059	0.006	0.00615
F	0.31	0.00315	0.0027	0.00315
G	0.10	0.00135	0.00105	0.00115
H	0.03	0	0	0

Yield (mg/mL): 0.576 Dilution Factor: 100×

Protein purified on 6/7/2022 and assayed on 6/8/2022.
Data uploaded by nikchupkin and last updated on 2022-07-14 16:58:39.

Design Data

Rosetta Score for R427L: 4.947

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.