D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F248N

Expression Data

Yield (mg/mL): 0.302

Kinetic Assay Data

**slope of F248N A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.7	5.5	9
B	25.00	4.5	4.5	4.4
C	8.33	3.5	3.8	3.6
D	2.78	2	2	2.3
E	0.93		1	1
F	0.31	0.4		0.5
G	0.10	0.3	0.3	0.3
H	0.03	0.4	0	0.3

Yield (mg/mL): 0.302 Dilution Factor: 10×

Protein purified on 7/18/2022 and assayed on 7/19/2022.
Data uploaded by morgan55 and last updated on 2022-07-20 14:38:12.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	306	309	275.7
B	25.00	256.3	240	275.4
C	8.33	230.8	211.8	231.2
D	2.78	105.7	115.3	128.6
E	0.93	43.8	44.3	47.1
F	0.31	16.1	14.7	17.3
G	0.10	5.5	5	6.3
H	0.03	0.7	0	0.3

Yield (mg/mL): 2.872 Dilution Factor: 100×

Protein purified on 7/18/2022 and assayed on 7/19/2022.
Data uploaded by morgan55 and last updated on 2022-07-20 14:35:54.

Design Data

Rosetta Score for F248N: 6.204

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.