D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M221K

Expression Data

Yield (mg/mL): 0.17

Kinetic Assay Data

**slope of M221K A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	56.6	57.8	54.7
B	25.00	43.4	43.4	39.8
C	8.33	26.8	28.3	27.7
D	2.78	12.3	14.4	13.2
E	0.93	4.6	4.7	4.5
F	0.31	1.8	1.9	1.7
G	0.10	0.8	0.6	1
H	0.03	1	1	0.7

Yield (mg/mL): 0.17 Dilution Factor: 10×

Protein purified on 7/18/2022 and assayed on 7/19/2022.
Data uploaded by morgan55 and last updated on 2022-07-20 15:05:18.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	306	309	275.7
B	25.00	256.3	240	275.4
C	8.33	230.8	211.8	231.2
D	2.78	105.7	115.3	128.6
E	0.93	43.8	44.3	47.1
F	0.31	16.1	14.7	17.3
G	0.10	5.5	5	6.3
H	0.03	0.7	0	0.3

Yield (mg/mL): 2.872 Dilution Factor: 100×

Protein purified on 7/18/2022 and assayed on 7/19/2022.
Data uploaded by morgan55 and last updated on 2022-07-20 14:35:54.

Design Data

Rosetta Score for M221K: 5.526

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.