D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q139K

Expression Data

Yield (mg/mL): 0.342

Kinetic Assay Data

**slope of Q139K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01370	0.01140	0.01235
B	25.00	0.007751	0.008750	0.007800
C	8.33	0.006050	0.006900	0.006000
D	2.78	0.005150	0.004800	0.004400
E	0.93	0.003200	0.002550	0.002750
F	0.31	0.001500	0.001650	0.001450
G	0.10	0.0007500	0.0008500	0.0008000
H	0.03	0	0.0002000	0.0003500

Yield (mg/mL): 0.342 Dilution Factor: 100×

Protein purified on 7/20/2022 and assayed on 7/20/2022.
Data uploaded by Nana-Awawu and last updated on 2022-07-27 13:49:04.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2450	0.2404	0.2372
B	25.00	0.2153	0.2000	0.2054
C	8.33	0.1507	0.1449	0.1483
D	2.78	0.09315	0.1166	0.1011
E	0.93	0.05545	0.06975	0.05970
F	0.31	0.02245		0.02270
G	0.10	0.008750		0.008600
H	0.03	0	0	0.0002500

Yield (mg/mL): 2.842 Dilution Factor: 100×

Protein purified on 7/20/2022 and assayed on 7/20/2022.
Data uploaded by Nana-Awawu and last updated on 2022-07-28 21:04:02.

Design Data

Rosetta Score for Q139K: -0.415

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.