D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant K61S

Expression Data

Yield (mg/mL): 0.452

Kinetic Assay Data

**slope of K61S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.01960	0.01980	0.01960
B	25.00	0.01480	0.01290	0.01235
C	8.33	0.01280	0.01245	0.01745
D	2.78	0.006700	0.007550	0.006400
E	0.93	0.003750	0.003650	0.003150
F	0.31	0.001700	0.001400	0.001350
G	0.10	0.0007000	0.0005500	0.0008000
H	0.03	0	0	0.0005500

Yield (mg/mL): 0.452 Dilution Factor: 100×

Protein purified on 7/19/22 and assayed on 7/20/22.
Data uploaded by bridget5 and last updated on 2022-08-01 15:31:39.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2450	0.2404	0.2372
B	25.00	0.2153	0.2000	0.2054
C	8.33	0.1507	0.1449	0.1483
D	2.78	0.09315	0.1166	0.1011
E	0.93	0.05545	0.06975	0.05970
F	0.31	0.02245		0.02270
G	0.10	0.008750		0.008600
H	0.03	0	0	0.0002500

Yield (mg/mL): 2.842 Dilution Factor: 100×

Protein purified on 7/20/2022 and assayed on 7/20/2022.
Data uploaded by Nana-Awawu and last updated on 2022-07-28 21:04:02.

Design Data

Rosetta Score for K61S: -0.035

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.