D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q313E

Expression Data

Yield (mg/mL): 4.2

Kinetic Assay Data

**slope of Q313E A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	507.4	522	500.2
B	25.00	357.3	353	349.7
C	8.33	282.7	276.8	285.3
D	2.78	162.5	186.2	172.6
E	0.93	71.1	87.2	74.7
F	0.31	0.1	28.9	46.7
G	0.10	12.2	11.8	10.9
H	0.03	0.2	0.3	0.1

Yield (mg/mL): 4.2 Dilution Factor: 100×

Protein purified on 3/27/2022 and assayed on 7/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:14:33.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	331.6	311.7	310
B	25.00	248.6	224.1	240.2
C	8.33	202.1	219.5	193.2
D	2.78	157	139.3	159
E	0.93	88.6	78.7	67.9
F	0.31	37.6	31.5	27
G	0.10	13.8	12.8	11.5
H	0.03	0.2	0.3	0.4

Yield (mg/mL): 2.6 Dilution Factor: 100×

Protein purified on 3/27/2022 and assayed on 7/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:12:28.

Thermostability Assay Data

**slope of Q313E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	45.0	0.001300	0.001200	0.001500
B	44.2	0.002700	0.002300	0.002600
C	42.9	0.01360	0.01500	0.01890
D	41.3	0.1911	0.1996	0.2143
E	38.9	1.007	1.052	1.013
F	36.8	1.258	1.215
G	35.6	1.332	1.281	1.288
H	35.0	1.534	1.451	1.513

Protein purified on 2/27/2022 and assayed on 2/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:15:23.

Reference Data for WT

**slope of Q313E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	45.0	0.0052	0.0052	0.0051
B	44.2	0.0149	0.0158
C	42.9	0.0685	0.093	0.1045
D	41.3	0.3375	0.4125	0.4341
E	38.9	1.187	0.9029	1.093
F	36.8	1.098	1.063	1.02
G	35.6	1.305	1.291	1.231
H	35.0	1.277	1.371	1.282

Protein purified on 2/27/2022 and assayed on 2/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:13:00.

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.