D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E388L

Yield (mg/mL): 3.79

**slope of E388L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.2255	0.2269	0.2292
B	25.00	0.1812	0.1792	0.1829
C	8.33	0.1457	0.1515	0.1571
D	2.78	0.1015	0.1013	0.09425
E	0.93	0.04485	0.04875	0.03925
F	0.31	0.01710	0.01950	0.01520
G	0.10	0.007500	0.008000	0.005750
H	0.03	0.003350	0.003150	0.002550

Yield (mg/mL): 3.79 Dilution Factor: 100×

Protein purified on 7/27/2022 and assayed on 7/28/2022.
Data uploaded by thomasa and last updated on 2022-07-28 20:57:36.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3500	0.3489	0.3449
B	25.00	0.2942	0.2738	0.2824
C	8.33	0.2351	0.2236	0.2178
D	2.78	0.1344	0.1513	0.1476
E	0.93	0.06125	0.06905	0.07145
F	0.31	0.02450	0.02915	0.02825
G	0.10	0.009350	0.01090	0.01130
H	0.03		0.004800	0.005000

Yield (mg/mL): 3.264 Dilution Factor: 100×

Protein purified on 7/27/2022 and assayed on 7/28/2022.
Data uploaded by thomasa and last updated on 2022-07-28 20:49:17.

**slope of E388L A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	35.0
B	35.6
C	36.8	0.7366	0.7535	0.7067
D	38.9	0.5405	0.5701	0.5214
E	41.3	0.09780	0.09900	0.1114
F	42.9	0.01120	0.01210
G	44.2	0.002600	0.002900	0.003600
H	45.0	0.001200	0.001100	0.001100

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by thomasa and last updated on 2022-07-28 21:19:27.

**slope of E388L A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	35.0
B	35.6
C	36.8	0.8227	0.5690	0.9194
D	38.9	0.9212	1.022	0.8387
E	41.3	0.4718	0.4307	0.4794
F	42.9	0.1115	0.1044
G	44.2	0.02490		0.02660
H	45.0	0.007900	0.008000	0

Protein purified on 7/27/22 and assayed on 7/28/22.
Data uploaded by thomasa and last updated on 2022-07-28 21:36:06.

The unpublished data above were collected by students from Crown College. For inquiries, please contact Aeisha Thomas: <thomasa@crown.edu>.