D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M221N

Expression Data

Yield (mg/mL): 1.5

Kinetic Assay Data

**slope of M221N A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	104.3	103.7	108.4
B	25.00	84.8	85.8	84.3
C	8.33	65.7	65.2	65.6
D	2.78	38.6	32.9	38.3
E	0.93	17.1	19.5	18.8
F	0.31	6.4	6.9	8
G	0.10	2.5	3.3	2.6
H	0.03	0.5	0.2	0.8

Yield (mg/mL): 1.5 Dilution Factor: 100×

Protein purified on 3/27/2022 and assayed on 7/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:18:42.

Reference Data for WT

**slope of WT A_{420 nm} (10⁻³ min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	331.6	311.7	310
B	25.00	248.6	224.1	240.2
C	8.33	202.1	219.5	193.2
D	2.78	157	139.3	159
E	0.93	88.6	78.7	67.9
F	0.31	37.6	31.5	27
G	0.10	13.8	12.8	11.5
H	0.03	0.2	0.3	0.4

Yield (mg/mL): 2.6 Dilution Factor: 100×

Protein purified on 3/27/2022 and assayed on 7/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:12:28.

Thermostability Assay Data

**slope of M221N A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	45.0	0.0006000	0	0.0006000
B	44.2	0.0006000	0.0005000	0
C	42.9	0.001100	0.0007000	0.0009000
D	41.3	0.004500	0.004300	0.004000
E	38.9	0.02170	0.02690	0.02740
F	36.8	0.06910	0.05710	0.06480
G	35.6	0.1302	0.1216	0.1257
H	35.0	0.1841	0.1934	0.1921

Protein purified on 2/27/2022 and assayed on 2/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:19:14.

Reference Data for WT

**slope of M221N A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	45.0	0.0052	0.0052	0.0051
B	44.2	0.0149	0.0158
C	42.9	0.0685	0.093	0.1045
D	41.3	0.3375	0.4125	0.4341
E	38.9	1.187	0.9029	1.093
F	36.8	1.098	1.063	1.02
G	35.6	1.305	1.291	1.231
H	35.0	1.277	1.371	1.282

Protein purified on 2/27/2022 and assayed on 2/28/2022.
Data uploaded by dkaluka and last updated on 2022-07-28 21:13:00.

Citations

The unpublished data above were collected by students from Taylor University. For inquiries, please contact Daniel Kaluka: <daniel_kaluka@taylor.edu>.