D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120T

Expression Data

Yield (mg/mL): 0.462

Kinetic Assay Data

**slope of W120T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0007000	0.0007000
B	25.00	0.0004000	0.0005000	0.0005500
C	8.33		0.0004500	0.0004500
D	2.78	0.0003500	0.0004000	0.0004500
E	0.93	0	0.0003500	0.0003000
F	0.31	0.0003000	0.0003500	0.0002500
G	0.10	0.0003500	0.0003000	0
H	0.03	0.0003000	0.0003000	0.0003500

Yield (mg/mL): 0.462 Dilution Factor: 10×

Protein purified on 8/1/2022 and assayed on 8/2/2022.
Data uploaded by anng19 and last updated on 2022-08-08 15:02:54.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.3910	0.3377
B	25.00		0.2788	0.2606
C	8.33	0.2535	0.2011	0.1958
D	2.78		0.1210	0.1086
E	0.93	0.03795	0.05940	0.04655
F	0.31	0.02700	0.02625	0.02090
G	0.10	0.007100	0.01110	0.009549
H	0.03	0.0004500	0.0003500	0.0005000

Yield (mg/mL): 3.592 Dilution Factor: 100×

Protein purified on 8/1/2022 and assayed on 8/2/2022.
Data uploaded by anng19 and last updated on 2022-08-08 14:30:29.

Design Data

Rosetta Score for W120T: -0.24

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.