D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N293S

Expression Data

Yield (mg/mL): 2.91

Kinetic Assay Data

**slope of N293S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00	0.002200	0.002400
C	8.33	0.001550	0.001650	0.001350
D	2.78	0.001550	0.001150	0.002700
E	0.93	0.001050	0.001100	0.001050
F	0.31	0.0007500	0.0009000	0.0008000
G	0.10	0.0005500	0.0005500	0.0006500
H	0.03	0	0.0003500	0

Yield (mg/mL): 2.91 Dilution Factor: 100×

Protein purified on 8/1/2022 and assayed on 8/2/2022.
Data uploaded by anng19 and last updated on 2022-08-08 15:08:49.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.3910	0.3377
B	25.00		0.2788	0.2606
C	8.33	0.2535	0.2011	0.1958
D	2.78		0.1210	0.1086
E	0.93	0.03795	0.05940	0.04655
F	0.31	0.02700	0.02625	0.02090
G	0.10	0.007100	0.01110	0.009549
H	0.03	0.0004500	0.0003500	0.0005000

Yield (mg/mL): 3.592 Dilution Factor: 100×

Protein purified on 8/1/2022 and assayed on 8/2/2022.
Data uploaded by anng19 and last updated on 2022-08-08 14:30:29.

Design Data

Rosetta Score for N293S: 0.493

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.