D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W125R

Expression Data

Yield (mg/mL): 0.184

Kinetic Assay Data

**slope of W125R A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.50E-02	2.22E-02	3.54E-02
B	25.00	2.67E-02	2.75E-02	3.08E-02
C	8.33	1.77E-02	2.26E-02	2.45E-02
D	2.78	1.62E-02	1.53E-02	1.70E-02
E	0.93		7.35E-03	7.85E-03
F	0.31	2.20E-03	2.60E-03	3.00E-03
G	0.10	1.15E-03	1.15E-03	1.45E-03
H	0.03	3.00E-04		3.00E-04

Yield (mg/mL): 0.184 Dilution Factor: 10×

Protein purified on 8/9/22 and assayed on 8/10/22.
Data uploaded by codliu and last updated on 2022-08-12 22:16:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	6.22E-02	6.67E-02	6.47E-02
B	25.00	5.83E-02	5.65E-02	5.68E-02
C	8.33	4.95E-02	4.40E-02	4.72E-02
D	2.78	2.61E-02	3.18E-02	2.85E-02
E	0.93	1.13E-02	1.25E-02	1.18E-02
F	0.31	3.40E-03	4.15E-03	4.30E-03
G	0.10	1.50E-03	1.90E-03	1.65E-03
H	0.03	0	3.00E-04	2.00E-04

Yield (mg/mL): 0.868 Dilution Factor: 100×

Protein purified on 8/9/22 and assayed on 8/10/22.
Data uploaded by codliu and last updated on 2022-08-12 22:22:54.

Thermostability Assay Data

**slope of W125R A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	4.25E-02	4.17E-02	4.44E-02
B	48.3	4.48E-02	4.48E-02	4.69E-02
C	45.7	4.40E-02	4.49E-02	4.81E-02
D	42.4	4.38E-02	4.66E-02	4.53E-02
E	37.7	4.53E-02	4.78E-02	5.05E-02
F	33.6	5.43E-02	5.82E-02	5.52E-02
G	31.3	6.24E-02	7.90E-02	6.81E-02
H	30.0	1.02E-01	1.08E-01	1.16E-01

Protein purified on 8/9/22 and assayed on 8/10/22.
Data uploaded by codliu and last updated on 2022-08-12 22:49:13.

Reference Data for WT

**slope of W125R A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	4.14E-02	4.29E-02	4.29E-02
B	48.3	4.48E-02	4.60E-02	5.06E-02
C	45.7	4.66E-02	5.10E-02	5.12E-02
D	42.4	5.56E-02	6.08E-02	6.25E-02
E	37.7	6.87E-02	7.50E-02	7.62E-02
F	33.6	9.45E-02	8.84E-02	8.95E-02
G	31.3	9.37E-02	1.00E-01	9.54E-02
H	30.0	1.29E-01	1.31E-01	1.30E-01

Protein purified on 8/9/22 and assayed on 8/11/22.
Data uploaded by codliu and last updated on 2022-08-12 22:51:55.

Design Data

Rosetta Score for W125R: 10.074

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.