Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.868

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	6.22E-02	6.67E-02	6.47E-02
	B	25.00	5.83E-02	5.65E-02	5.68E-02
	C	8.33	4.95E-02	4.40E-02	4.72E-02
	D	2.78	2.61E-02	3.18E-02	2.85E-02
	E	0.93	1.13E-02	1.25E-02	1.18E-02
	F	0.31	3.40E-03	4.15E-03	4.30E-03
	G	0.10	1.50E-03	1.90E-03	1.65E-03
	H	0.03	0	3.00E-04	2.00E-04

Yield (mg/mL): 0.868     Dilution Factor:  100×

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Protein purified on 8/9/22 and assayed on 8/10/22.
Data uploaded by codliu and last updated on 2022-08-12 22:22:54.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.14E-02	4.29E-02	4.29E-02
	B	48.3	4.48E-02	4.60E-02	5.06E-02
	C	45.7	4.66E-02	5.10E-02	5.12E-02
	D	42.4	5.56E-02	6.08E-02	6.25E-02
	E	37.7	6.87E-02	7.50E-02	7.62E-02
	F	33.6	9.45E-02	8.84E-02	8.95E-02
	G	31.3	9.37E-02	1.00E-01	9.54E-02
	H	30.0	1.29E-01	1.31E-01	1.30E-01

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Protein purified on 8/9/22 and assayed on 8/11/22.
Data uploaded by codliu and last updated on 2022-08-12 22:51:55.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.