D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L108E

Expression Data

Yield (mg/mL): 3.698

Kinetic Assay Data

**slope of L108E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4219	0.3980	0.4277
B	25.00	0.3805	0.3674	0.3877
C	8.33	0.3162	0.3123	0.2871
D	2.78	0.1574	0.1562	0.1786
E	0.93	0.07830	0.07535	0.07775
F	0.31	0.02940	0.02750	0.03225
G	0.10	0.01015	0.009651	0.01160
H	0.03	0.0002500	0.0002000	0.0003500

Yield (mg/mL): 3.698 Dilution Factor: 100×

Protein purified on 8/29/2022 and assayed on 8/30/2022.
Data uploaded by cgyeow and last updated on 2022-09-13 12:38:11.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.6285	0.5793	0.5627
B	25.00	0.4523	0.4218	0.4231
C	8.33	0.3396	0.3309	0.3122
D	2.78	0.1889	0.2053	0.1883
E	0.93	0.07200	0.08010	0.07240
F	0.31	0.03060	0.03560	0.03265
G	0.10	0.009000	0.01010	0.01205
H	0.03	0.0002500	0.0002500	0.0002500

Yield (mg/mL): 4.238 Dilution Factor: 100×

Protein purified on 8/29/2022 and assayed on 8/30/2022.
Data uploaded by cgyeow and last updated on 2022-09-13 12:42:14.

Design Data

Rosetta Score for L108E: -1.785

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.