Raw Data for BglB Variant Y265F

Expression Data

SDS-PAGE
 0.302

Kinetic Assay Data

slope of Y265F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.006300 0.004400 0.003050
B 25.00 0.002800 0.002650
C 8.33 0.002450 0.001750 0.001800
D 2.78 0.001450 0.001300 0.001300
E 0.93 0.0008000 0.0006000 0.0007000
F 0.31 0.0004000 0.0004000 0.0004000
G 0.10 0.0003000 0.0004000 0
H 0.03 0.0002000 0.0003000 0.0004000
 0.302      10×

Protein purified on 10/8/22 and assayed on 10/10/22.
Data uploaded by codliu and last updated on 2022-10-14 16:53:17.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2487 0.2448
B 25.00 0.2198 0.2134 0.2515
C 8.33 0.1798 0.1969 0.2210
D 2.78 0.1025 0.1342 0.1278
E 0.93 0.04515 0.06875 0.05835
F 0.31 0.02140 0.02630 0.02135
G 0.10 0.008550 0.007550 0.009100
H 0.03 0 0.0003000 0
 2.242      100×

Protein purified on 10/8/22 and assayed on 10/10/22.
Data uploaded by codliu and last updated on 2022-10-14 16:24:09.


Thermostability Assay Data

slope of Y265F A420 nm (min−1)
T (°C) 1 2 3
A 30.0 0.006300 0.004400 0.003050
B 31.3 0.002800 0.002650
C 33.6 0.001750 0.001800
D 37.7 0.001450 0.001300 0.001300
E 42.4 0.0008000 0.0006000 0.0007000
F 45.7 0.0004000 0.0004000 0.0004000
G 48.3 0.0003000 0.0004000 0
H 50.0 0.0002000 0.0003000 0.0004000

Protein purified on 10/8/22 and assayed on 10/9/22.
Data uploaded by codliu and last updated on 2022-10-14 16:54:08.


Reference Data for WT

slope of Y265F A420 nm (min−1)
T (°C) 1 2 3
A 30.0 0.2487 0.2448
B 31.3 0.2198 0.2134 0.2515
C 33.6 0.1798 0.1969 0.2210
D 37.7 0.1025 0.1342 0.1278
E 42.4 0.04515 0.06875 0.05835
F 45.7 0.02140 0.02630 0.02135
G 48.3 0.008550 0.007550 0.009100
H 50.0 0 0.0003000 0

Protein purified on 10/8/22 and assayed on 10/9/22.
Data uploaded by codliu and last updated on 2022-10-14 16:35:58.


Design Data

-0.565

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.