D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L171F

Expression Data

Yield (mg/mL): 2.7

Kinetic Assay Data

**slope of L171F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.08E-01	2.20E-01	2.20E-01
B	25.00	1.90E-01	1.96E-01	1.95E-01
C	8.33	1.55E-01	1.60E-01	1.69E-01
D	2.78	1.33E-01	1.43E-01	1.39E-01
E	0.93	8.18E-02	8.35E-02	7.38E-02
F	0.31	3.45E-02	3.61E-02	3.20E-02
G	0.10	1.65E-02	1.60E-02	1.15E-02
H	0.03	2.98E-03	0	0

Yield (mg/mL): 2.7 Dilution Factor: 100×

Protein purified on 04-10-2022 and assayed on 05-10-2022.
Data uploaded by ReetS and last updated on 2022-10-27 15:53:40.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.78E-01	1.84E-01	2.03E-01
B	25.00	1.53E-01	1.65E-01	1.69E-01
C	8.33	1.32E-01	1.24E-01	1.24E-01
D	2.78	7.45E-02	7.41E-02	8.29E-02
E	0.93	3.50E-02	3.63E-02	4.34E-02
F	0.31	1.34E-02	1.32E-02	1.58E-02
G	0.10	5.38E-03	5.40E-03	6.50E-03
H	0.03	1.52E-05	0	3.70E-05

Yield (mg/mL): 2.905 Dilution Factor: 100×

Protein purified on 04-10-2022 and assayed on 05-10-2022.
Data uploaded by ReetS and last updated on 2022-10-27 15:22:31.

Design Data

Rosetta Score for L171F: 1.616

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.