D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A145C

Expression Data

Yield (mg/mL): 2.416

Kinetic Assay Data

**slope of A145C A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.65E-01	2.63E-01	2.55E-01
B	25.00	2.26E-01	2.18E-01	2.08E-01
C	8.33	1.65E-01	1.68E-01	1.79E-01
D	2.78	1.21E-01	1.12E-01	1.23E-01
E	0.93	5.02E-02	4.69E-02	4.98E-02
F	0.31	1.73E-02	1.73E-02	1.67E-02
G	0.10	6.23E-03	6.17E-03	5.97E-03
H	0.03	0	1.70E-05	2.00E-05

Yield (mg/mL): 2.416 Dilution Factor: 100×

Protein purified on 20-10-2022 and assayed on 21-10-2022.
Data uploaded by ReetS and last updated on 2022-10-27 16:18:16.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.38E-01	2.32E-01	2.39E-01
B	25.00	1.90E-01	1.99E-01	1.97E-01
C	8.33	1.65E-01	1.58E-01	1.62E-01
D	2.78	9.05E-02	1.03E-01	1.12E-01
E	0.93	4.21E-02	4.57E-02	4.39E-02
F	0.31	1.62E-02	1.63E-02	1.56E-02
G	0.10	5.66E-03	5.62E-03	5.93E-03
H	0.03	2.00E-05	2.24E-05	2.12E-05

Yield (mg/mL): 2.482 Dilution Factor: 100×

Protein purified on 20-10-2022 and assayed on 21-10-2022.
Data uploaded by ReetS and last updated on 2022-10-27 16:27:14.

Design Data

Rosetta Score for A145C: 2.966

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.