D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A80S

Expression Data

Yield (mg/mL): 2.042

Kinetic Assay Data

**slope of A80S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.35E-01	2.57E-01	2.40E-01
B	25.00	2.03E-01	2.10E-01	2.09E-01
C	8.33	1.83E-01	1.81E-01	1.47E-01
D	2.78	1.34E-01	1.03E-01	1.30E-01
E	0.93	5.32E-02	4.91E-02	5.68E-02
F	0.31	2.01E-02	1.66E-02	1.98E-02
G	0.10	7.62E-03	5.93E-03	7.37E-03
H	0.03	1.52E-05	0	2.73E-05

Yield (mg/mL): 2.042 Dilution Factor: 100×

Protein purified on 20-10-2022 and assayed on 21-10-2022.
Data uploaded by ReetS and last updated on 2022-10-30 18:20:38.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.38E-01	2.32E-01	2.39E-01
B	25.00	1.90E-01	1.99E-01	1.97E-01
C	8.33	1.65E-01	1.58E-01	1.62E-01
D	2.78	9.05E-02	1.03E-01	1.12E-01
E	0.93	4.21E-02	4.57E-02	4.39E-02
F	0.31	1.62E-02	1.63E-02	1.56E-02
G	0.10	5.66E-03	5.62E-03	5.93E-03
H	0.03	2.00E-05	2.24E-05	2.12E-05

Yield (mg/mL): 2.482 Dilution Factor: 100×

Protein purified on 20-10-2022 and assayed on 21-10-2022.
Data uploaded by ReetS and last updated on 2022-10-27 16:27:14.

Design Data

Rosetta Score for A80S: 2.785

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.