Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.98

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	6.11E-02	6.53E-02	6.93E-02
	B	25.00	5.83E-02	6.20E-02	7.00E-02
	C	8.33	4.64E-02	4.80E-02	4.93E-02
	D	2.78	2.75E-02	2.94E-02	2.73E-02
	E	0.93	1.30E-02	1.25E-02	1.18E-02
	F	0.31	5.11E-03	5.63E-03	4.36E-03
	G	0.10	1.86E-03	1.96E-03	1.77E-03
	H	0.03	2.73E-05	1.45E-05	2.30E-05

Yield (mg/mL): 0.98     Dilution Factor:  100×

Download these data

Protein purified on 11/9/2022 and assayed on 11/9/2022.
Data uploaded by ShreyaT and last updated on 2022-11-16 11:20:51.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	5.00E-04	5.00E-04	6.00E-04
	B	48.3	3.00E-04	4.00E-04	5.00E-04
	C	45.7	7.00E-04	5.00E-04	6.00E-04
	D	42.4	4.60E-03	4.60E-03	4.10E-03
	E	37.7	2.13E-02		2.11E-02
	F	33.6	1.94E-02	1.44E-02	1.64E-02
	G	31.3	1.89E-02	2.03E-02	1.97E-02
	H	30.0	1.56E-02	1.80E-02	2.00E-02

Download these data

Protein purified on 11/9/2022 and assayed on 11/9/2022.
Data uploaded by ShreyaT and last updated on 2022-11-16 11:36:44.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.