Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.79

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.1927	0.1925	0.2163
	B	25.00	0.1763	0.1585	0.1588
	C	8.33	0.1211	0.1249	0.1223
	D	2.78	0.0744	0.06635
	E	0.93	0.02975	0.02955	0.0315
	F	0.31	0.01185	0.01125
	G	0.10	0.00555	0.0049	0.0055
	H	0.03		0.0018	0.00195

Yield (mg/mL): 1.79     Dilution Factor:  100×

Download these data

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by jeremiah and last updated on 2022-11-16 11:40:17.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0007	0.0007	0.0007
	B	48.3	0.0005	0.0004
	C	45.7	0.0011	0.0012
	D	42.4	0.0286	0.0312	0.0321
	E	37.7	0.1001	0.1097	0.1059
	F	33.6	0.112	0.1139	0.112
	G	31.3	0.1265	0.0987	0.1287
	H	30.0	0.1035	0.1305

Download these data

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by jeremiah and last updated on 2022-11-16 12:05:29.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.