Raw Data for BglB Variant Q124I

Expression Data

Yield (mg/mL): 1.74

Kinetic Assay Data

slope of Q124I A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0778	0.07845	0.0735
	B	25.00	0.0688	0.06365	0.0707
	C	8.33	0.0591	0.0561	0.05065
	D	2.78	0.03715	0.0363
	E	0.93	0.0175	0.0158	0.01505
	F	0.31	0.00735	0.00765	0.0068
	G	0.10	0.00245	0.00185	0.00265
	H	0.03	0.0011	0.00135	0.001

Yield (mg/mL): 1.74     Dilution Factor:  100×

Download these data

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by vater and last updated on 2022-11-18 16:47:28.

Reference Data for WT

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.06225	0.06555	0.06175
	B	25.00	0.05945	0.0579	0.05405
	C	8.33	0.05055	0.05005	0.0477
	D	2.78	0.03315	0.03645	0.0303
	E	0.93	0.0148	0.01515	0.0143
	F	0.31	0.0065	0.00605	0.00605
	G	0.10	0.0024	0.0024	0.00215
	H	0.03	0.00125	0.00105	0.001

Yield (mg/mL): 0.604     Dilution Factor:  100×

Download these data

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by vater and last updated on 2022-11-16 14:56:26.

Thermostability Assay Data

slope of Q124I A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.0008000	0	0.0007000
	B	48.3	0.0008001	0.001100	0.0006000
	C	45.7	0.0008000	0.001000	0.001200
	D	42.4	0.01360	0.01370
	E	37.7	0.02350	0.03200	0.02850
	F	33.6		0.03560	0.03720
	G	31.3	0.03700	0.03190	0.02500
	H	30.0	0.03290	0.02200	0.02810

Download these data

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by ksmcbride2004 and last updated on 2022-11-17 17:26:49.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Design Data

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.