D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q124M

Expression Data

Yield (mg/mL): 0.83

Kinetic Assay Data

**slope of Q124M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.75E-02	4.76E-02	4.19E-02
B	25.00	4.25E-02	3.91E-02	3.90E-02
C	8.33
D	2.78	2.66E-02	2.66E-02	2.86E-02
E	0.93	1.69E-02	1.80E-02	1.59E-02
F	0.31	6.75E-03	8.00E-03	7.05E-03
G	0.10	3.05E-03	3.05E-03	2.70E-03
H	0.03	1.25E-03	1.35E-03	1.10E-03

Yield (mg/mL): 0.83 Dilution Factor: 100×

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by dsingh and last updated on 2022-11-18 13:13:59.

Thermostability Assay Data

**slope of Q124M A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	7.00E-04	7.00E-04	7.00E-04
B	48.3
C	45.7	6.00E-04	5.00E-04	0
D	42.4
E	37.7	6.80E-03	8.10E-03	7.70E-03
F	33.6	1.12E-02	1.17E-02	1.15E-02
G	31.3	1.54E-02	1.49E-02	1.79E-02
H	30.0	1.65E-02	1.64E-02	1.66E-02

Protein purified on 11/2/22 and assayed on 11/9/22.
Data uploaded by dsingh and last updated on 2022-11-18 13:18:32.

Design Data

Rosetta Score for Q124M: 5.791

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.