D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220E

Expression Data

Yield (mg/mL): 4.371

Kinetic Assay Data

**slope of N220E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.02775	0.03105	0.03285
B	25.00	0.01710	0.01845	0.02010
C	8.33	0.009150	0.009450	0.009450
D	2.78	0.004350	0.004650	0.004200
E	0.93	0.001950	0.001950	0.001950
F	0.31	0.001050	0.0009000	0.0009000
G	0.10	0.0007500	0.0006000	0.0006000
H	0.03	0.001050	0	0.0006000

Yield (mg/mL): 4.371 Dilution Factor: 100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by GabrielaRamos and last updated on 2022-11-18 07:49:34.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.04E-01	3.80E-01	3.70E-01
B	25.00	3.16E-01	3.03E-01	3.26E-01
C	8.33	2.55E-01	2.54E-01	2.48E-01
D	2.78	2.22E-01	2.19E-01	2.24E-01
E	0.93	1.29E-01	1.11E-01	1.13E-01
F	0.31	3.99E-02	3.93E-02	3.63E-02
G	0.10	1.65E-02	1.40E-02	2.01E-02
H	0.03	0	0	4.50E-04

Yield (mg/mL): 9.881 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-12-02 10:32:31.

Thermostability Assay Data

**slope of N220E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0	0	0.0004500
B	48.3	0.0004501	0.0006000	0
C	45.7	0.0006000	0	0.0007502
D	42.4	0.0006000	0.0004500	0
E	37.7	0.001200		0.001200
F	33.6	0.003600	0.003750	0.003900
G	31.3	0.004800	0.005250	0.004650
H	30.0	0.004500	0.004800	0.004650

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by GabrielaRamos and last updated on 2022-11-18 08:15:54.

Reference Data for WT

**slope of N220E A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.0	6.00E-04	0	4.50E-04
C	46.0	6.00E-04	1.05E-03	7.50E-04
D	42.0	6.60E-03	6.45E-03	5.85E-03
E	38.0	3.74E-02	3.84E-02	3.56E-02
F	34.0	4.25E-02	4.16E-02	4.13E-02
G	31.0	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.

Design Data

Rosetta Score for N220E: 5.607

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.