Raw Data for BglB Variant N220A
Expression Data
7.953
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 1.65E-01 | 1.65E-01 | 1.74E-01 | ||
| B | 25.00 | 1.75E-01 | 1.33E-01 | 1.34E-01 | ||
| C | 8.33 | 8.73E-02 | 8.16E-02 | 8.33E-02 | ||
| D | 2.78 | 3.77E-02 | 3.57E-02 | 3.62E-02 | ||
| E | 0.93 | 1.38E-02 | 1.52E-02 | 1.37E-02 | ||
| F | 0.31 | 5.55E-03 | 5.55E-03 | 5.55E-03 | ||
| G | 0.10 | 2.55E-03 | 2.40E-03 | 2.55E-03 | ||
| H | 0.03 | 6.00E-04 | 4.50E-04 | 6.00E-04 |
Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by 6281254 and last updated on 2024-08-08 18:42:28.
Reference Data for WT
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 4.04E-01 | 3.80E-01 | 3.70E-01 | ||
| B | 25.00 | 3.16E-01 | 3.03E-01 | 3.26E-01 | ||
| C | 8.33 | 2.55E-01 | 2.54E-01 | 2.48E-01 | ||
| D | 2.78 | 2.22E-01 | 2.19E-01 | 2.24E-01 | ||
| E | 0.93 | 1.29E-01 | 1.11E-01 | 1.13E-01 | ||
| F | 0.31 | 3.99E-02 | 3.93E-02 | 3.63E-02 | ||
| G | 0.10 | 1.65E-02 | 1.40E-02 | 2.01E-02 | ||
| H | 0.03 | 0 | 0 | 4.50E-04 |
Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-12-02 10:32:31.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 1.80E-03 | 6.00E-04 | 0 | ||
| B | 48.8 | 4.50E-04 | 0 | 6.00E-04 | ||
| C | 46.0 | 0 | 0 | 0 | ||
| D | 42.0 | 1.35E-03 | 1.35E-03 | 1.05E-03 | ||
| E | 38.0 | 3.71E-02 | 3.86E-02 | 3.57E-02 | ||
| F | 34.0 | 5.66E-02 | 5.42E-02 | 4.44E-02 | ||
| G | 31.0 | 5.21E-02 | 5.48E-02 | 4.98E-02 | ||
| H | 30.0 | 5.67E-02 | 5.64E-02 | 5.31E-02 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 6.00E-04 | 7.50E-04 | 6.00E-04 | ||
| B | 48.0 | 6.00E-04 | 0 | 4.50E-04 | ||
| C | 46.0 | 6.00E-04 | 1.05E-03 | 7.50E-04 | ||
| D | 42.0 | 6.60E-03 | 6.45E-03 | 5.85E-03 | ||
| E | 38.0 | 3.74E-02 | 3.84E-02 | 3.56E-02 | ||
| F | 34.0 | 4.25E-02 | 4.16E-02 | 4.13E-02 | ||
| G | 31.0 | 4.98E-02 | 5.04E-02 | 4.80E-02 | ||
| H | 30.0 | 4.10E-02 | 4.35E-02 | 3.98E-02 |
Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.
Design Data
Citations
The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.