Raw Data for BglB Variant N220V

Expression Data

SDS-PAGE
 1.304

Kinetic Assay Data

slope of N220V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.04275 0.045 0.04635
B 25.00 0.0387 0.039 0.03705
C 8.33 0.02595 0.0285 0.02805
D 2.78 0.0192 0.0204 0.021
E 0.93 0.0144 0.01245 0.015
F 0.31 0.0075 0.006 0.0081
G 0.10 0.00345 0.00285 0.0036
H 0.03 0.00075 0.00075 0.0009
 1.304      100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by ariellah01 and last updated on 2022-11-18 07:47:56.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 4.04E-01 3.80E-01 3.70E-01
B 25.00 3.16E-01 3.03E-01 3.26E-01
C 8.33 2.55E-01 2.54E-01 2.48E-01
D 2.78 2.22E-01 2.19E-01 2.24E-01
E 0.93 1.29E-01 1.11E-01 1.13E-01
F 0.31 3.99E-02 3.93E-02 3.63E-02
G 0.10 1.65E-02 1.40E-02 2.01E-02
H 0.03 0 0 4.50E-04
 9.881      100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-12-02 10:32:31.


Thermostability Assay Data

slope of N220V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.00075
B 48.3 0.0009 0.0006
C 45.7 0.0009001 0.00105
D 42.4 0.00075 0.00105 0.0009
E 37.7 0.0027 0.00255 0.00285
F 33.6 0.0075 0.0069 0.0069
G 31.3 0.0081 0.00855 0.0084
H 30.0 0.0069 0.00735 0.0071

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by ariellah01 and last updated on 2023-02-08 12:44:28.


Reference Data for WT

slope of N220V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 6.00E-04 7.50E-04 6.00E-04
B 48.0 6.00E-04 0 4.50E-04
C 46.0 6.00E-04 1.05E-03 7.50E-04
D 42.0 6.60E-03 6.45E-03 5.85E-03
E 38.0 3.74E-02 3.84E-02 3.56E-02
F 34.0 4.25E-02 4.16E-02 4.13E-02
G 31.0 4.98E-02 5.04E-02 4.80E-02
H 30.0 4.10E-02 4.35E-02 3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.


Design Data

54

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.