D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E177T

Yield (mg/mL): 2.726

**slope of E177T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.174741	0.168245	0.165628
B	18.75	0.12485	0.117422	0.115666
C	4.69	0.0690776	0.066343	0.0648327
D	1.17	0.0306564	0.028223	0.0279679
E	0.29	0.00627455	0.00539939	0.00591758
F	0.07	0.00262848	0.00249636	0.00247455
G	0.02	0.000666667	0.000629091	0.00062
H	0.00	0.000218788	1.93E-04	2.01E-04

Yield / A_{280 nm}: 2.726 Dilution Factor: 100×

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.83E-02	3.83E-02	3.67E-02
B	18.75	2.87E-02	2.90E-02	2.75E-02
C	4.69	1.81E-02	1.85E-02	1.75E-02
D	1.17	8.66E-03	7.97E-03	6.91E-03
E	0.29	2.80E-03	2.45E-03	2.24E-03
F	0.07	8.14E-04	6.82E-04	6.20E-04
G	0.02	2.75E-04	2.06E-04	2.08E-04
H	0.00	8.12E-05	6.67E-05	7.21E-05

Yield / A_{280 nm}: 0.207 Dilution Factor: 50×

Protein purified on 9/21/19 and assayed on 9/21/19.
Data uploaded by peishanh and last updated on 2022-06-27 07:04:03.

The above data have been published in the following journal article(s):

• Huang, P.; Chu, S.K.; Frizzo, H.N.; Connolly, M.P.; Caster, R.W.; & Siegel, J. B. (2020) “Evaluating Protein Engineering Thermostability Prediction Tools Using an Independently Generated Dataset..” ACS Omega 5(12), 6487–6493.