Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.294

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.13E-01	1.10E-01	1.11E-01
	B	25.00	9.09E-02	8.88E-02	9.15E-02
	C	8.33	6.57E-02	6.83E-02	6.77E-02
	D	2.78	3.86E-02	3.65E-02	3.95E-02
	E	0.93	2.24E-02	2.40E-02	2.28E-02
	F	0.31	1.02E-02	9.75E-03	1.02E-02
	G	0.10	4.80E-03	4.20E-03	5.25E-03
	H	0.03	6.00E-04		7.50E-04

Yield (mg/mL): 2.294     Dilution Factor:  100×

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Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:08:58.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	6.00E-04	7.50E-04	6.00E-04
	B	48.3	6.00E-04		4.50E-04
	C	45.7	6.00E-04	1.05E-03	7.50E-04
	D	42.4	6.60E-03	6.45E-03	5.85E-03
	E	37.7	3.74E-02	3.84E-02	3.56E-02
	F	33.6	4.25E-02	4.16E-02	4.13E-02
	G	31.3	4.98E-02	5.04E-02	4.80E-02
	H	30.0	4.10E-02	4.35E-02	3.98E-02

Download these data

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:16:50.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.