D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178N

Yield (mg/mL): 0.0464

**slope of H178N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.004057		0.004125
B	25.00	0.003423	0.004132	0.003552
C	8.33	0.002823	0.002864	0.002959
D	2.78	0.002427	0.0021	0.001957
E	0.93	0.00152	0.001405	0.001555
F	0.31		0.001098	0.001159
G	0.10	0.000961	0.001186	0.001561
H	0.03	0.001145	0.001623

Yield (mg/mL): 0.0464 Dilution Factor: 100×

Protein purified on 10/7/22 and assayed on 10/28/22.
Data uploaded by gmoreno1 and last updated on 2022-11-18 13:05:06.

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.07	0.09	0.07
B	25.00	0.06	0.06	0.06
C	8.33	0.04	0.04	0.04
D	2.78	0.03	0.03	0.03
E	0.93	0.01	0.01
F	0.31	0.006048	0.006286	0.0063
G	0.10	0.002959	0.002768	0.003075
H	0.03	0.001711	0.001057	0.000456818

Yield (mg/mL): 0.309 Dilution Factor: 100×

Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by enielse and last updated on 2022-11-22 16:29:10.

Data missing or deleted from the database.

**slope of H178N A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	0.001671	0.001303
B	48.3	0.002391		0.002151
C	45.7	0.001303		0.001294
D	42.4	0.002546	0.003746	0.003154
E	37.7		0.02	0.02
F	33.6	0.03	0.03	0.03
G	31.3		0.04	0.04
H	30.0		0.04	0.04

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:47:10.

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.