Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.309

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.09	0.1	0.09
	B	25.00	0.06	0.06	0.07
	C	8.33	0.05	0.05	0.04
	D	2.78	0.03	0.03	0.03
	E	0.93	0.01	0.01	0.01
	F	0.31	0.005393	0.006307	0.004977
	G	0.10	0.002707	0.002741	0.003457
	H	0.03	0.000988636	0.001036

Yield (mg/mL): 0.309     Dilution Factor:  100×

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Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by enielse and last updated on 2022-11-22 16:27:41.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.002597	0.002306	0.002177
	B	48.3	0.002854	0.003137
	C	45.7	0.003377	0.00318
	D	42.4	0.003411	0.00372	0.003891
	E	37.7	0.02	0.02	0.02
	F	33.6		0.03	0.03
	G	31.3	0.03	0.03	0.03
	H	30.0	0.03	0.03

Download these data

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:39:14.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.