Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.309

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.09	0.1	0.09
	B	25.00	0.07	0.06	0.06
	C	8.33	0.05	0.05	0.05
	D	2.78	0.03	0.03	0.03
	E	0.93		0.01	0.01
	F	0.31	0.006586	0.005952	0.005693
	G	0.10	0.003252	0.003259	0.002836
	H	0.03	0.000920455	0.000968182

Yield (mg/mL): 0.309     Dilution Factor:  100×

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Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by enielse and last updated on 2022-11-22 16:31:57.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	0.002409	0.002743	0.003557
	B	48.3	0.00228		0.00246
	C	45.7	0.002974	0.002409	0.004294
	D	42.4	0.003746	0.0039
	E	37.7		0.03	0.03
	F	33.6		0.04	0.04
	G	31.3	0.03	0.03	0.03
	H	30.0	0.04	0.04	0.05

Download these data

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:55:09.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.