D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S302Y

Expression Data

Yield (mg/mL): 11.07

Kinetic Assay Data

**slope of S302Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.59E-01	4.99E-01	5.18E-01
B	25.00	3.62E-01	3.98E-01	3.94E-01
C	8.33	2.86E-01	2.87E-01	3.07E-01
D	2.78	1.81E-01	1.82E-01	1.84E-01
E	0.93	7.89E-02	8.22E-02	8.57E-02
F	0.31	3.60E-02	3.77E-02	3.33E-02
G	0.10	1.34E-02	1.23E-02	1.25E-02
H	0.03	0	4.50E-04	0

Yield (mg/mL): 11.07 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Danielle and last updated on 2022-11-18 12:43:38.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3587	0.3504	0.3458
B	25.00	0.2868	0.3096	0.3092
C	8.33	0.2193	0.2441	0.2356
D	2.78	0.1266	0.1298	0.1290
E	0.93	0.05670	0.05880	0.05925
F	0.31	0.02340	0.02235	0.02235
G	0.10	0.008250	0.008400	0.008250
H	0.03		0.001050	0.001050

Yield (mg/mL): 6.878 Dilution Factor: 100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by nnaja003 and last updated on 2022-12-01 15:45:42.

Thermostability Assay Data

**slope of S302Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	0	4.50E-04
B	48.3	0	7.50E-04	6.00E-04
C	45.7	7.50E-04	6.00E-04	6.00E-04
D	42.4	1.80E-02	1.94E-02	2.22E-02
E	37.7	2.01E-01	2.05E-01	2.17E-01
F	33.6	2.26E-01	2.32E-01	2.35E-01
G	31.3	2.29E-01	2.33E-01	2.28E-01
H	30.0	2.24E-01	2.27E-01	2.18E-01

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Danielle and last updated on 2022-11-18 12:48:11.

Reference Data for WT

**slope of S302Y A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.0	6.00E-04	0	4.50E-04
C	46.0	6.00E-04	1.05E-03	7.50E-04
D	42.0	6.60E-03	6.45E-03	5.85E-03
E	38.0	3.74E-02	3.84E-02	3.56E-02
F	34.0	4.25E-02	4.16E-02	4.13E-02
G	31.0	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by Seaotter01 and last updated on 2022-11-18 08:12:14.

Design Data

Rosetta Score for S302Y: 5.757

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.