D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S302K

Expression Data

Yield (mg/mL): 6.905

Kinetic Assay Data

**slope of S302K A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.05E-01	3.95E-01	3.89E-01
B	25.00	3.14E-01	3.23E-01	2.67E-01
C	8.33	2.40E-01	2.42E-01	2.43E-01
D	2.78	1.42E-01	1.37E-01	1.41E-01
E	0.93	6.35E-02	6.32E-02	6.29E-02
F	0.31	2.34E-02	2.13E-02	2.21E-02
G	0.10	9.75E-03	8.55E-03	9.45E-03
H	0.03	6.00E-04	0	9.00E-04

Yield (mg/mL): 6.905 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by kvazq032 and last updated on 2022-11-18 12:34:26.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3069	0.3233	0.3195
B	25.00	0.2660	0.2668	0.2742
C	8.33	0.1985	0.2022	0.2011
D	2.78	0.1112	0.1280	0.1002
E	0.93	0.04740	0.05040	0.05835
F	0.31	0.02115	0.01935	0.01905
G	0.10	0.007050	0.006900	0.007650
H	0.03	0.0006000	0.0006000	0

Yield (mg/mL): 6.509 Dilution Factor: 100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by Ocapu001 and last updated on 2022-11-18 12:34:22.

Thermostability Assay Data

**slope of S302K A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	6.00E-04	6.00E-04
B	48.3	9.00E-04	6.00E-04	7.50E-04
C	45.7		7.50E-04	7.50E-04
D	42.4	2.85E-03	2.85E-03	2.85E-03
E	37.7	1.16E-01	1.19E-01	1.14E-01
F	33.6	1.23E-01	1.28E-01	1.15E-01
G	31.3	1.90E-01	1.88E-01	1.78E-01
H	30.0	1.74E-01	1.80E-01	1.63E-01

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by kvazq032 and last updated on 2024-08-01 14:47:45.

Reference Data for WT

**slope of S302K A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.3	6.00E-04		4.50E-04
C	45.7	6.00E-04	1.05E-03	7.50E-04
D	42.4	6.60E-03	6.45E-03	5.85E-03
E	37.7	3.74E-02	3.84E-02	3.56E-02
F	33.6	4.25E-02	4.16E-02	4.13E-02
G	31.3	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:16:50.

Design Data

Rosetta Score for S302K: 9.819

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.