D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S302F

Expression Data

Yield (mg/mL): 9.031

Kinetic Assay Data

**slope of S302F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.03E-01	3.14E-01	2.93E-01
B	25.00	2.46E-01	2.53E-01	2.23E-01
C	8.33	1.96E-01	2.05E-01	2.08E-01
D	2.78	1.44E-01	1.63E-01	1.43E-01
E	0.93	7.83E-02	8.51E-02	9.05E-02
F	0.31	4.01E-02	4.95E-02	4.28E-02
G	0.10	2.07E-02	1.95E-02	2.61E-02
H	0.03	0	7.50E-04	6.00E-04

Yield (mg/mL): 9.031 Dilution Factor: 100×

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by afern672 and last updated on 2022-11-18 12:34:06.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3587	0.3504	0.3458
B	25.00	0.2868	0.3096	0.3092
C	8.33	0.2193	0.2441	0.2356
D	2.78	0.1266	0.1298	0.1290
E	0.93	0.05670	0.05880	0.05925
F	0.31	0.02340	0.02235	0.02235
G	0.10	0.008250	0.008400	0.008250
H	0.03		0.001050	0.001050

Yield (mg/mL): 6.878 Dilution Factor: 100×

Protein purified on 10/21/2022 and assayed on 10/28/2022.
Data uploaded by nnaja003 and last updated on 2022-12-01 15:45:42.

Thermostability Assay Data

**slope of S302F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	0	4.50E-04
B	48.3	0	7.50E-04	7.50E-04
C	45.7	7.50E-04	6.00E-04	7.50E-04
D	42.4	5.25E-03	5.40E-03	4.95E-03
E	37.7	1.19E-01	1.22E-01	1.23E-01
F	33.6	1.50E-01	1.45E-01	1.47E-01
G	31.3	1.54E-01	1.47E-01	1.49E-01
H	30.0	1.47E-01	1.45E-01	1.44E-01

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by afern672 and last updated on 2022-11-18 12:40:40.

Reference Data for WT

**slope of S302F A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0	6.00E-04	7.50E-04	6.00E-04
B	48.3	6.00E-04		4.50E-04
C	45.7	6.00E-04	1.05E-03	7.50E-04
D	42.4	6.60E-03	6.45E-03	5.85E-03
E	37.7	3.74E-02	3.84E-02	3.56E-02
F	33.6	4.25E-02	4.16E-02	4.13E-02
G	31.3	4.98E-02	5.04E-02	4.80E-02
H	30.0	4.10E-02	4.35E-02	3.98E-02

Protein purified on 10/21/22 and assayed on 10/28/22.
Data uploaded by mayoral and last updated on 2022-11-18 08:16:50.

Design Data

Rosetta Score for S302F: 6.165

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.