Raw Data for BglB Variant T352V

Expression Data

SDS-PAGE
 0.045

Kinetic Assay Data

slope of T352V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.00E-02 9.36E-03 1.00E-02
B 25.00 1.00E-02 1.00E-02 1.00E-02
C 8.33 7.35E-03 9.16E-03 8.11E-03
D 2.78 5.86E-03 4.57E-03 4.30E-03
E 0.93 2.47E-03 2.99E-03 2.13E-03
F 0.31 1.53E-03 1.71E-03
G 0.10 1.71E-03 1.75E-03 1.96E-03
H 0.03 5.80E-04 1.02E-03 1.18E-03
 0.045      10×

Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by vlignos and last updated on 2022-11-18 13:09:52.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.09 0.1 0.09
B 25.00 0.06 0.06 0.07
C 8.33 0.05 0.05 0.04
D 2.78 0.03 0.03 0.03
E 0.93 0.01 0.01 0.01
F 0.31 0.005393 0.006307 0.004977
G 0.10 0.002707 0.002741 0.003457
H 0.03 0.000988636 0.001036
 0.309      100×

Protein purified on 10/7/2022 and assayed on 10/21/2022.
Data uploaded by enielse and last updated on 2022-11-22 16:27:41.


Thermostability Assay Data

Data missing or deleted from the database.


Reference Data for WT

slope of T352V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.002597 0.002306 0.002177
B 48.3 0.002854 0.003137
C 45.7 0.003377 0.00318
D 42.4 0.003411 0.00372 0.003891
E 37.7 0.02 0.02 0.02
F 33.6 0.03 0.03
G 31.3 0.03 0.03 0.03
H 30.0 0.03 0.03

Protein purified on 10/7/2022 and assayed on 10/28/2022.
Data uploaded by enielse and last updated on 2022-11-18 11:39:14.


Design Data

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.